Secretory expression and purification of Bacillus licheniformis keratinase in insect cells
نویسندگان
چکیده
منابع مشابه
Secretory expression and purification of Bacillus licheniformis keratinase in insect cells
The keratinase (kerA) gene from Bacillus licheniformis PWD-1 was expressed and purified in insect cells. First, the sequence encoding Ker-His-Flag was designed based on the amino acid sequence of the protein and peptide and codon optimization in order to ensure the high expression in insect cells. In the next step, the synthetic DNA was inserted into the pUC57 vector and then sub-cloned in the ...
متن کاملExpression Optimization and Purification of Glutamate Endopeptidase from halo-thermo tolerant Bacillus licheniformis SL-1
Aims and Background: Glutamate-specific endopeptidase (GSE, EC 3.4.21.19) is belonging to the serine protease family enzymes. A glutamate-specific endopeptidase has the ability to cleavage peptide bonds for the protein structure analysis, solid phase synthesis of peptides and preparation of nanotubes. The purpose of this investigation was to produce glutamate endopeptidase enzyme from the Bacil...
متن کاملCloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
Background: VP4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. VP4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. Methods: Simian rotavirus SA11 gene 4 cDNA was cloned into a cloning plasmid pDONRTM by recombination reaction using clonase II enzyme mix. The resulting clone was c...
متن کاملPurification Properties of Bacillus thuringiensis TS2 Keratinase Enzyme
This study was focused on purification and characterization of keratinase from Bacillus thuringiensis TS2, isolated from feather dumping soil site. Zymogram of crude enzyme on native-PAGE presented bands with keratinase activity of molecular weights is 41 kDa. In purification steps 2.69 fold purification was achieved after 80% ammonium precipitation of the with 34.42% recovery. In further purif...
متن کاملSCREENING AND CHARACTERIZATION OF KERATINASE FROM Bacillus licheniformis ISOLATED FROM NAMAKKAL POULTRY FARM
Keratin are insoluble fibrous proteins found in hair, wool, feather, nail, horns and other epithelial covering which is rich in beta helical coil linked through cysteine bridges. Keratinase (EC 3.4.4.25) belongs to the class hydrolase which are able to hydrolyse insoluble keratins more efficient than other proteases. The bacteria Bacillus licheniformis showing higher keratinase activity was scr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLOS ONE
سال: 2017
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0183764